Application

α-Bungarotoxin-tetramethylrhodamine from Bungarus multicinctus (Formosan Banded Krait) has been used in immunofluorescence staining (1:200) for identification of neuromuscular junction in experimental models. It has also been used to block muscle contraction in myotome cells.

α-Bungarotoxin-tetramethylrhodamine from Bungarus multicinctus was used at a dilution of 1:220 to identify the distribution of nicotinic acetylcholine receptors at neuromuscular junctions of longitudinal sections of rat gastrocnemius muscle.

Biochem/physiol Actions

Useful for detecting the distribution of nicotinic acetylcholine receptors at neuromuscular junctions.

α-Bungarotoxin is a high-affinity antagonist for nicotinic acetylcholine receptors (AChRs) from muscle but not the neurons. Labeling α-Bungarotoxin with tetramethylrhodamine enables the detection of bungarotoxin-binding receptors at neuromuscular junctions.